Donkey normal serum (10 ml)
Recommended dilution: 2-5% (v/v) serum in PBS/TBS. To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.For a Western Blot
Rabbit Ig fraction
Rabbit immunoglobulin fraction is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.005 % sodium azide is added as preservative.Concentration is 4.5 mg/ml.Rabbit serum can be used
Agrisera IncuBlocker (rabbit antibody)
Kit components: Wash Buffer (Reagent A) and the "all in one" goat anti-rabbit HRP-conjugated secondary antibody in advanced blocking solution (Reagent B).Required: primary antibody of your choice and