S100A1/S100B polyclonal antibody
产品名称： S100A1/S100B polyclonal antibody
英文名称： S100A1/S100B polyclonal antibody
产品编号： PAB11341
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against full length recombinant S100A1/S100B.
- Immunogen:
- Recombinant protein corresponding to full length bovine S100A1/S100B.
- Host:
- Rabbit
- Reactivity:
- Bovine, Dog, Mouse, Rat
- Specificity:
- A 98% homology is noted for S100 alpha chain from primate sources. Mouse, rat and dog show 94% homology with the bovine S100 alpha sequence. Expect cross reactivity with S100 from most mammalian sources. Homologies for the S100 beta chain are similar.
- Form:
- Lyophilized
- Storage Buffer:
- Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot after reconstitution to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
- Recommend Usage:
- ELISA (1:5000-1:25000)
Western Blot (1:500-1:3000)
Immunohistochemistry (1:200-1:2000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Endogenous morphine-like compound immunoreactivity increases in parkinsonism.
Charron G, Doudnikoff E, Laux A, Berthet A, Porras G, Canron MH, Barroso-Chinea P, Li Q, Qin C, Nosten-Bertrand M, Giros B, Delalande F, Van Dorsselaer A, Vital A, Goumon Y, Bezard E.Brain. 2011 Aug;134(Pt 8):2321-38. Epub 2011 Jul 8.
- 2.
- S100 beta is a target protein of neurocalcin delta, an abundant isoform in glial cells.
Okazaki K, Obata NH, Inoue S, Hidaka H.Biochem J. 1995 Mar 1;306 ( Pt 2):551-5.
- 3.
- Molecular cloning of cDNA of S100 alpha subunit mRNA.
Kuwano R, Maeda T, Usui H, Araki K, Yamakuni T, Ohshima Y, Kurihara T, Takahashi Y.FEBS Lett. 1986 Jun 23;202(1):97-101.
- 4.
- Ions binding to S100 proteins: structural changes induced by calcium and zinc on S100a and S100b proteins.
Baudier J, Gerard D.Biochemistry. 1983 Jul 5;22(14):3360-9.
- Applications
- Western Blot
- Western blot using S100A1/S100B polyclonal antibody (Cat # PAB11341) shows detection of a band ~11 KDa corresponding to bovine S100A1/S100B monomer (100 ng loaded, arrowhead lane 1).
The antibody also detects S100A1/S100B from rat brain lysate (Lane 2). Approximately 35 ug of a rat brain whole cell lysate was separated by 16% SDS-PAGE and transferred onto nitrocellulose.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunohistochemistry of S100A1/S100B polyclonal antibody (Cat # PAB11341) was used at a 1:500 dilution to detect S100A1/S100B by immunohistochemistry in human brain astrocyte tumor tissue.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication,Alan Yen, Life Span Biosciences, Seattle, WA.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunohistochemistry of S100A1/S100B polyclonal antibody (Cat # PAB11341) was used at a 1:500 dilution to detect S100A1/S100B.
Dark nuclear staining is observed within basket cells located near the Purkinje cells in the mouse cerebellum.
Personal Communication, Teri Johnson, Stowers Institute, Kansas City, MO.
- Entrez GeneID:
- 528735
- Protein Accession#:
- P02639 (Bovine gene ID : 528735)
- Gene Name:
- S100A1
- Gene Alias:
- MGC159706
- Gene Description:
- S100 calcium binding protein A1
- Gene Ontology:
- Hyperlink
- Other Designations:
- -
- Entrez GeneID:
- 525716
- Protein Accession#:
- P02639 (Bovine gene ID : 528735)
- Gene Name:
- S100B
- Gene Alias:
- MGC128275
- Gene Description:
- S100 calcium binding protein B
- Gene Ontology:
- Hyperlink
- Other Designations:
- S100 protein, beta polypeptide